Dipak Maskey , PhD

Research Associate, Henry Ford Health System

Location: DETROIT

Department: Pharmacology & Toxicology

maskeydi@msu.edu

Employment

Instructor, Henry Ford Hospital, Detroit, 2017 - Present

Publications

Supplemental Fig. S3 Systolic blood pressure measurement of AV NKCC2-Cas9 control and AV NKCC2-Cas9 +AAV gRNA ACTN4 transduced rats. Quantification of systolic blood pressure of AV NKCC2-Cas9 control and AV NKCC2-Cas9 +AAV gRNA ACTN4 transduced rats as measured under isoflurane anesthesia with catheter placed inside the femoral artery. Error bars represent ±S.E.*, (P<0.04, one-way ANOVA, n=4) AV NKCC2-Cas9 +AAV gRNA ACTN4 versus AV NKCC2-Cas9-control. (2024)

Supplemental Fig. S4. Measurement of body weight of rats after silencing of ACTN4 with shRNA/CRISPR-Cas9 system. A. Quantification of body weight after silencing of ACTN4 by shRNA. Error bars represent ±S.E.*, (P<0.97, unpaired t test, two-tailed, n=5) versus scrambled-shRNA. B. Quantification of body weight after silencing of ACTN4 by gRNA against ACTN4. Error bars represent ±S.E.*, (P<0.61, one-way ANOVA, n=5) versus Cas9-control. (2024)

Supplemental Fig. S5. Specificity of ACTN4 antibody was tested from Santa Cruz Biotechnology. Representative western blot shows single band of ACTN4 and ACTN4 expression is reduced by 60% in mTAL sample after silencing of ACTN4 with gRNA against ACTN4 as compared to control. (Exposure time: 30 sec.). (2024)

Supplemental Fig. S6. Expression of ACTN4 and NKCC2 in the renal cortex. Representative images showing Immunofluorescence labeling of a rat kidney cortex section for negative control (no primary antibody), and for ACTN4 (red) and NKCC2 (green) in paraffin embedded rat kidney sections, indicating expression of ACTN4 and NKCC2 in TALs as well as prominent ACTN4 labeling in glomerular podocytes (n=3). Scale bar, 20 um. (2024)

Supplemental fig S2: Identification of most effective gRNA against ACTN4 in Cas9-Neuroblastoma Neuro 2a cells. Representative blot showing expression of ACTN4 in Cas9 expressing Neuroblastoma Neuro 2a cells with 4 different gRNA sequences against ACTN4 (n=3). (Blot exposure time: 30 sec.). (2024)

Supplemental figure S1: Identification of most effective siRNA sequence for ACTN4 in NRK52E cells. Representative blot showing expression of ACTN4 in gene silencing by 4 different siRNA sequences for ACTN4 in transduced NRK52E (n=3). (Blot exposure time: 30 sec.). (2024)

The FSGS protein actinin-4 interacts with NKCC2 to regulate thick ascending limb NaCl reabsorption American Journal of Physiology-Renal Physiology (2024)