Sing Lee, PhD
he/his/him
Associate Professor
Pharmacology & Toxicology, Chemistry
Associate Professor
Pharmacology & Toxicology, Chemistry
Lab Staff
Lab Postdocs
Colleges
Associations
- Chemistry
Programs
Sections
- PHM 802 Cellular, Molecular and Integrated Systems Pharmacology (in-person) - Spring
- PHM 809 Drug Discovery and Medicinal Chemistry (in-person) - Spring
Education
- Ph.D., Chemistry, Michigan State University (2003-07-01—2012-01-01)
- Bachelor of Science, Chemistry, Hong Kong University of Science and Technology (2000-09-01—2003-06-01)
Funding
- Identifying the Receptors of Environmentally Sensitive Epoxy-Eicosanoids with AMS, National Institute of Environmental Health Sciences (2015-01-01—2016-12-30)
- Identifying the Receptors of Environmentally Sensitive Epoxy-Eicosanoids with AMS, National Institute of Environmental Health Sciences (2015-01-01—2016-12-30)
- Identifying the Receptors of Environmentally Sensitive Epoxy-Eicosanoids with AMS, National Institute of Environmental Health Sciences (2015-01-01—2016-12-30)
- Kinetics-Driven Drug Discovery Using Persistent Homology, Rare-Event Molecular Dynamics and Experimental Data, Directorate for Mathematical & Physical Sciences (2018-07-15—2022-06-30)
Works
- Quantitative Profiling Method for Oxylipins in Neurodegenerative Diseases by Liquid Chromatography Coupled with Tandem Mass Spectrometry. (2023-10-03)
- Cytochrome P450 and Epoxide Hydrolase Metabolites in Aβ and tau-induced Neurodegeneration: Insights fromCaenorhabditis elegans. (2023-10-02)
- Relative Importance of Soluble and Microsomal Epoxide Hydrolases for the Hydrolysis of Epoxy-Fatty Acids in Human Tissues. International Journal of Molecular Sciences (2021-05-08)
- Movement to the Clinic of Soluble Epoxide Hydrolase Inhibitor EC5026 as an Analgesic for Neuropathic Pain and for Use as a Nonaddictive Opioid Alternative. Journal of Medicinal Chemistry (2021-02-25)
- Cytochrome P450 Metabolism of Polyunsaturated Fatty Acids and Neurodegeneration. Nutrients (2020-11-16)
- Preparation and evaluation of soluble epoxide hydrolase inhibitors with improved physical properties and potencies for treating diabetic neuropathic pain. Bioorganic & Medicinal Chemistry (2020)
- Inactivation of Cys 674 in SERCA2 increases BP by inducing endoplasmic reticulum stress and soluble epoxide hydrolase. British Journal of Pharmacology (2020-01-30)
- Asymmetric Total Synthesis of 19,20-Epoxydocosapentaenoic Acid, a Bioactive Metabolite of Docosahexaenoic Acid. The Journal of Organic Chemistry (2019-12-06)
- Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids. Journal of Visualized Experiments (2019-06-28)
- Brain oxylipin concentrations following hypercapnia/ischemia: effects of brain dissection and dissection time. J Lipid Res (2019)
- Drug-Target Residence Time Affects in Vivo Target Occupancy through Multiple Pathways. ACS Cent Sci (2019)
- Targeted Metabolomics Identifies the Cytochrome P450 Monooxygenase Eicosanoid Pathway as a Novel Therapeutic Target of Colon Tumorigenesis. Cancer Res (2019)
- trans, trans-2,4-decadienal, a lipid peroxidation product, induces inflammatory responses via Hsp90- or 14–3-3ζ-dependent mechanisms. The Journal of Nutritional Biochemistry (2019)
- trans, trans-2,4-Decadienal, a lipid peroxidation product, induces inflammatory responses via Hsp90- or 14–3-3ζ-dependent mechanisms. The Journal of Nutritional Biochemistry (2020)
- Enzymatic synthesis and chemical inversion provide both enantiomers of bioactive epoxydocosapentaenoic acids. J Lipid Res (2018)
- Soluble epoxide hydrolase inhibition decreases reperfusion injury after focal cerebral ischemia. Sci Rep (2018)
- A new sensitive LC/MS/MS analysis of vitamin D metabolites using a click derivatization reagent, 2-nitrosopyridine. J Lipid Res (2017)
- Active-Site Flexibility and Substrate Specificity in a Bacterial Virulence Factor: Crystallographic Snapshots of an Epoxide Hydrolase. Structure (2017)
- Chemical synthesis and biological evaluation of omega-hydroxy polyunsaturated fatty acids. Bioorg Med Chem Lett (2017)
- Cyclooxygenase-derived proangiogenic metabolites of epoxyeicosatrienoic acids. Proc Natl Acad Sci U S A (2017)
- Cytochrome P450 monooxygenase lipid metabolites are significant second messengers in the resolution of choroidal neovascularization. Proc Natl Acad Sci U S A (2017)
- Endothelial Nox4-based NADPH oxidase regulates atherosclerosis via soluble epoxide hydrolase. Biochim Biophys Acta Mol Basis Dis (2017)
- Epoxide metabolites of arachidonate and docosahexaenoate function conversely in acute kidney injury involved in GSK3beta signaling. Proc Natl Acad Sci U S A (2017)
- Epoxy fatty acids mediate analgesia in murine diabetic neuropathy. Eur J Pain (2017)
- Inhibition of soluble epoxide hydrolase augments astrocyte release of vascular endothelial growth factor and neuronal recovery after oxygen-glucose deprivation. J Neurochem (2017)
- Linoleic acid participates in the response to ischemic brain injury through oxidized metabolites that regulate neurotransmission. Sci Rep (2017)
- Probing the orientation of inhibitor and epoxy-eicosatrienoic acid binding in the active site of soluble epoxide hydrolase. Arch Biochem Biophys (2017)
- Soluble Epoxide Hydrolase Inhibitor Attenuates Lipopolysaccharide-Induced Acute Lung Injury and Improves Survival in Mice. Shock (2017)
- Soluble Epoxide Hydrolase Pharmacological Inhibition Decreases Alveolar Bone Loss by Modulating Host Inflammatory Response, RANK-Related Signaling, Endoplasmic Reticulum Stress, and Apoptosis. J Pharmacol Exp Ther (2017)
- Soluble epoxide hydrolase in podocytes is a significant contributor to renal function under hyperglycemia. Biochim Biophys Acta Gen Subj (2017)
- Synthesis of cyclooxygenase metabolites of 8,9-epoxyeicosatrienoic acid (EET): 11- and 15-hydroxy 8,9-EETs. Org Biomol Chem (2017)
- omega-3 Polyunsaturated fatty acids and their cytochrome P450-derived metabolites suppress colorectal tumor development in mice. J Nutr Biochem (2017)
- Cytochrome P450 Oxidase 2C Inhibition Adds to omega-3 Long-Chain Polyunsaturated Fatty Acids Protection Against Retinal and Choroidal Neovascularization. Arterioscler Thromb Vasc Biol (2016)
- Ingestion of the epoxide hydrolase inhibitor AUDA modulates immune responses of the mosquito, Culex quinquefasciatus during blood feeding. Insect Biochem Mol Biol (2016)
- Molecular Mechanisms and New Treatment Paradigm for Atrial Fibrillation. Circ Arrhythm Electrophysiol (2016)
- Novel Omega-3 Fatty Acid Epoxygenase Metabolite Reduces Kidney Fibrosis. Int J Mol Sci (2016)
- Pro-atherogenic role of smooth muscle Nox4-based NADPH oxidase. J Mol Cell Cardiol (2016)
- Soluble Epoxide Hydrolase Inhibition and Epoxyeicosatrienoic Acid Treatment Improve Vascularization of Engineered Skin Substitutes. Plast Reconstr Surg Glob Open (2016)
- Soluble epoxide hydrolase inhibitor 1-trifluoromethoxyphenyl-3- (1-propionylpiperidin-4-yl) urea attenuates bleomycin-induced pulmonary fibrosis in mice. Cell Tissue Res (2016)
- "Turn-on" protein fluorescence: in situ formation of cyanine dyes. J Am Chem Soc (2015)
- "Turn-On" Protein Fluorescence: In Situ Formation of Cyanine Dyes. Journal of the American Chemical Society (2015)
- Endoplasmic reticulum stress in the peripheral nervous system is a significant driver of neuropathic pain. Proc Natl Acad Sci U S A (2015)
- Identification of potent inhibitors of the chicken soluble epoxide hydrolase. Bioorg Med Chem Lett (2015)
- Method for Sorting and Pairwise Selection of Nanobodies for the Development of Highly Sensitive Sandwich Immunoassays. Anal Chem (2015)
- PPARdelta signaling mediates the cytotoxicity of DHA in H9c2 cells. Toxicol Lett (2015)
- PPAR delta signaling mediates the cytotoxicity of DHA in H9c2 cells. Toxicology Letters (2015)
- An omega-3 epoxide of docosahexaenoic acid lowers blood pressure in angiotensin-II-dependent hypertension. J Cardiovasc Pharmacol (2014)
- An Omega-3 Epoxide of Docosahexaenoic Acid Lowers Blood Pressure in Angiotensin-II-Dependent Hypertension. Journal of Cardiovascular Pharmacology (2014)
- Effect of soluble epoxide hydrolase polymorphism on substrate and inhibitor selectivity and dimer formation. J Lipid Res (2014)
- Optimized inhibitors of soluble epoxide hydrolase improve in vitro target residence time and in vivo efficacy. J Med Chem (2014)
- Optimized Inhibitors of Soluble Epoxide Hydrolase Improve in Vitro Target Residence Time and in Vivo Efficacy. Journal of Medicinal Chemistry (2014)
- Symmetric adamantyl-diureas as soluble epoxide hydrolase inhibitors. Bioorg Med Chem Lett (2014)
- Epoxy fatty acids and inhibition of the soluble epoxide hydrolase selectively modulate GABA mediated neurotransmission to delay onset of seizures. PLoS One (2013)
- Epoxy Fatty Acids and Inhibition of the Soluble Epoxide Hydrolase Selectively Modulate GABA Mediated Neurotransmission to Delay Onset of Seizures. Plos One (2013)
- Epoxy metabolites of docosahexaenoic acid (DHA) inhibit angiogenesis, tumor growth, and metastasis. Proc Natl Acad Sci U S A (2013)
- Forster resonance energy transfer competitive displacement assay for human soluble epoxide hydrolase. Anal Biochem (2013)
- Rational design of a colorimetric pH sensor from a soluble retinoic acid chaperone. J Am Chem Soc (2013)
- Rational Design of a Colorimetric pH Sensor from a Soluble Retinoic Acid Chaperone. Journal of the American Chemical Society (2013)
- Unique mechanistic insights into the beneficial effects of soluble epoxide hydrolase inhibitors in the prevention of cardiac fibrosis. Proc Natl Acad Sci U S A (2013)
- Probing Wavelength Regulation with an Engineered Rhodopsin Mimic and a C15-Retinal Analogue. Chempluschem (2012)
- Tuning the electronic absorption of protein-embedded all-trans-retinal. Science (2012)
- Tuning the Electronic Absorption of Protein-Embedded All-trans-Retinal. Science (2012)
- Dissection of the critical binding determinants of cellular retinoic acid binding protein II by mutagenesis and fluorescence binding assay. Proteins (2009)
- Elucidating the exact role of engineered CRABPII residues for the formation of a retinal protonated Schiff base. Proteins (2009)
- Remarkable axial ligand effect on regioselectivity towards terminal alkenes in epoxidation of dienes by a robust manganese porphyrin. Chemical Communications (2003-02-20)
Employment
- Assistant Professor, Michigan State University (2017-01-01)
- Project Scientist, University of California Davis (2014-01-18—2016-12-31)
- Post-doctoral Scholar, University of California Davis (2010-03-01—2014-01-18)